Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Ssu72

Cell type

Cell type Class
Liver
Cell type
Hepatocytes
MeSH Description
The main structural component of the LIVER. They are specialized EPITHELIAL CELLS that are organized into interconnected plates called lobules.

Attributes by original data submitter

Sample

source_name
hepatocyte
strain
C57BL/6
cell type
hepatocyte
developmental stage
Purified hepatocytes of 6weeks mice
virus infection
Cre recombinase adenovirus infection
genotype
Ssu72 KO
chip antibody
Ssu72 (Cell Signaling, #12816)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and protein-chromatin complexes were isolated with antibody. Libraries were prepared according to Illumina's instructions accompanying the DNA Sample Kit and sequenced on the Genome Analyzer following the manufacturer's protocols.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

mm10

Number of total reads
112972447
Reads aligned (%)
73.1
Duplicates removed (%)
41.2
Number of peaks
2834 (qval < 1E-05)

mm9

Number of total reads
112972447
Reads aligned (%)
72.4
Duplicates removed (%)
40.7
Number of peaks
3425 (qval < 1E-05)

Base call quality data from DBCLS SRA