Antigen

Antigen Class

TFs and others

Antigen

Ssu72

Cell type

Cell type Class

Liver

Cell type

Hepatocytes

MeSH Description

The main structural component of the LIVER. They are specialized EPITHELIAL CELLS that are organized into interconnected plates called lobules.

Sample

source_name

hepatocyte

strain

C57BL/6

cell type

hepatocyte

developmental stage

Purified hepatocytes of 6weeks mice

virus infection

Cre recombinase adenovirus infection

genotype

Ssu72 KO

chip antibody

Ssu72 (Cell Signaling, #12816)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and protein-chromatin complexes were isolated with antibody. Libraries were prepared according to Illumina's instructions accompanying the DNA Sample Kit and sequenced on the Genome Analyzer following the manufacturer's protocols.

Sequencing Platform

instrument_model

Illumina HiSeq 2500

mm10

Number of total reads

112972447

Reads aligned (%)

73.1

Duplicates removed (%)

41.2

Number of peaks

2834 (qval < 1E-05)

mm9

Number of total reads

112972447

Reads aligned (%)

72.4

Duplicates removed (%)

40.7

Number of peaks

3425 (qval < 1E-05)